Identification of a New Wide-Compatibility Locus in Inter-Subspecific Hybrids of Rice (Oryza sativa L.)

Identification of a New Wide-Compatibility Locus in Inter-Subspecific Hybrids of Rice (Oryza sativa L.)

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As a special class of rice germplasm, wide-compatibility varieties (WCVs) guarantee the fertility of hybrids when there is cross-fertilization between two subspecies.In this study, Chenghui9348 was identified as a new member of the WCV family that improves pollen fertility in an inter-subspecific hybrid.Cytological analysis showed that the abnormal mitosis of microspores resulted in the sterility of pollens at the early bicellular stage in the inter-subspecific hybrid.

Furthermore, the new F12 locus, corresponding to improvements MANUKA HONEY 250+MGO in fertility of the indica-japonica hybrid, was found to co-segregate with the RM1047 marker and associated with a region of approximately 630 kb flanked by the D1101 and D1164 markers on chromosome 12.In this region, two putative genes were predicted as the candidates KIDS ELDERBERRY COUGH SYRUP for wide-compatibility genes (WCGs).Sequence analysis revealed that, compared with indica/japonica alleles, deletion/insertion occurred within exons of both putative genes.

Together, the present study identified another new WC locus, F12, and offers more opportunities for further exploitation of inter-subspecific hybrids in rice.